Basic laboratory methods for biotechnology / Lisa A.Seidman  

Public ISBD Titre : Basic laboratory methods for biotechnology : Textbook and laboratory reference Type de document : texte imprimé Auteurs : Lisa A.Seidman, Auteur ; Cynthia J.Moore, Auteur ; Jeanette Mowery, Auteur Mention d'édition : 3rd edition Editeur : CRC Press Année de publication : 2022 Importance : 1171p. Présentation : ill. Format : 28 cmx21cm ISBN/ISSN/EAN : 978-0-367-24488-0 Langues : Anglais (eng) Langues originales : Anglais (eng) Index. décimale : 660 Technologie chimique (chimie industrielle) et techniques connexes Résumé : . Basic Laboratory Methods for Biotechnology, Third Edition is a versatile textbook that provides students with a solid foundation to pursue employment in the biotech industry and can later serve as a practical reference to ensure success at each stage in their career. The authors focus on basic principles and methods while skillfully including recent innovations and industry trends throughout. Fundamental laboratory skills are emphasized, and boxed content provides step by step laboratory method instructions for ease of reference at any point in the students’ progress. Worked through examples and practice problems and solutions assist student comprehension. Coverage includes safety practices and instructions on using common laboratory instruments. Key Features: Provides a valuable reference for laboratory professionals at all stages of their careers. Focuses on basic principles and methods to provide students with the knowledge needed to begin a career in the Biotechnology industry. Describes fundamental laboratory skills. Includes laboratory scenario-based questions that require students to write or discuss their answers to ensure they have mastered the chapter content. Updates reflect recent innovations and regulatory requirements to ensure students stay up to date. Tables, a detailed glossary, practice problems and solutions, case studies and anecdotes provide students with the tools needed to master the content. Note de contenu : . Contente: UNIT I Biotechnology is the Transformation of Knowledge into Useful Products Chapter 1. Techniques to Manipulate DNA: The Root of the Biotechnology Industry Chapter 2. The Biotechnology Industry Branches Out Chapter 3. The Business of Biotechnology: The Transformation of Knowledge into Products UNIT II Introduction to Quality in Biotechnology Workplaces Chapter 4. An Overview of Quality Principles in Biotechnology. Chapter 5. Quality in Research Laboratories. Chapter 6. Documentation: The Foundation of Quality UNIT III Safety in the Laboratory Chapter 7. Introduction to a Safe Workplace. Chapter 8.Working Safely in the Laboratory: General Considerations and Physical Hazards Chapter 9.Working Safely with Chemicals. Chapter 10.Working Safely with Biological Materials UNIT IV Math in the Biotechnology Laboratory: An Overview Chapter 11. Basic Math Techniques. Chapter 12.Proportional Relationships. Chapter 13.Relationships and Graphing. Chapter 14.Descriptions of Data: Descriptive Statistics UNIT V Obtaining Reproducible Laboratory Measurements. Chapter 15.Introduction to Instrumental Methods and Electricity. Chapter 16.The Measurement of Weight Chapter 17.The Measurement of Volume. Chapter 18.The Measurement of Temperature. Chapter 19.The Measurement of pH, Selected Ions, and Conductivity. Chapter 20.Measurements Involving Light – Part A: Basic Principles and Instrumentation UNIT VI Laboratory Solutions. Chapter 21.Preparation of Laboratory Solutions – Part A: Concentration Expressions and Calculations. Chapter 22.Preparation of Laboratory Solutions – Part B: Basic Procedures and Buffers. Chapter 23.Solutions: Associated Procedures and Information. Chapter 24.Laboratory Solutions to Support the Activity of Biological Macromolecules UNIT VII Quality Assays and Tests. Chapter 25.Introduction to Quality Laboratory Tests and Assays. Chapter 26.Achieving Reproducible Results with Polymerase Chain Reaction Assays Chapter 27.Measurements Involving Light – Part B: Assays. Chapter 28.Achieving Reproducible Immunoassay Results. UNIT VIII Cell Culture and Reproducibility Chapter 29.Introduction to Quality Practices for Cell Culture. Chapter 30.Culture Media for Intact Cells. Culture and Reproducibility Chapter 31.Introduction to Quality Practices for Cell Culture Chapter 32.Culture Media for Intact Cells UNIT IX Basic Separation Method Chapter 33.ntroduction to Filtration. Chapter 34.Introduction to Centrifugation Chapter 35.Introduction to Bioseparations UNIT X Biotechnology and Regulatory Affairs. Chapter 36.Biotechnology and the Regulation of Medical and Food Products. Chapter 37.The Lifecycles and Regulation of Pharmaceutical Products. Chapter 38.The Lifecycles and Regulation of Biopharmaceutical and Regenerative Medicine Products. Chapter 39.Quality Systems in a Regulated Production Facility. Appendix: Answers to Practice Problems. Acronyms. Glossary Terms. En ligne : https://images.routledge.com/common/jackets/crclarge/978036724/9780367244880.jpg Basic laboratory methods for biotechnology : Textbook and laboratory reference [texte imprimé] / Lisa A.Seidman, Auteur ; Cynthia J.Moore, Auteur ; Jeanette Mowery, Auteur  . -  3rd edition . - [S.l.] : CRC Press, 2022 . - 1171p. : ill. ; 28 cmx21cm. ISBN : 978-0-367-24488-0 Langues : Anglais (eng) Langues originales : Anglais (eng) Index. décimale : 660 Technologie chimique (chimie industrielle) et techniques connexes Résumé : . Basic Laboratory Methods for Biotechnology, Third Edition is a versatile textbook that provides students with a solid foundation to pursue employment in the biotech industry and can later serve as a practical reference to ensure success at each stage in their career. The authors focus on basic principles and methods while skillfully including recent innovations and industry trends throughout. Fundamental laboratory skills are emphasized, and boxed content provides step by step laboratory method instructions for ease of reference at any point in the students’ progress. Worked through examples and practice problems and solutions assist student comprehension. Coverage includes safety practices and instructions on using common laboratory instruments. Key Features: Provides a valuable reference for laboratory professionals at all stages of their careers. Focuses on basic principles and methods to provide students with the knowledge needed to begin a career in the Biotechnology industry. Describes fundamental laboratory skills. Includes laboratory scenario-based questions that require students to write or discuss their answers to ensure they have mastered the chapter content. Updates reflect recent innovations and regulatory requirements to ensure students stay up to date. Tables, a detailed glossary, practice problems and solutions, case studies and anecdotes provide students with the tools needed to master the content. Note de contenu : . Contente: UNIT I Biotechnology is the Transformation of Knowledge into Useful Products Chapter 1. Techniques to Manipulate DNA: The Root of the Biotechnology Industry Chapter 2. The Biotechnology Industry Branches Out Chapter 3. The Business of Biotechnology: The Transformation of Knowledge into Products UNIT II Introduction to Quality in Biotechnology Workplaces Chapter 4. An Overview of Quality Principles in Biotechnology. Chapter 5. Quality in Research Laboratories. Chapter 6. Documentation: The Foundation of Quality UNIT III Safety in the Laboratory Chapter 7. Introduction to a Safe Workplace. Chapter 8.Working Safely in the Laboratory: General Considerations and Physical Hazards Chapter 9.Working Safely with Chemicals. Chapter 10.Working Safely with Biological Materials UNIT IV Math in the Biotechnology Laboratory: An Overview Chapter 11. Basic Math Techniques. Chapter 12.Proportional Relationships. Chapter 13.Relationships and Graphing. Chapter 14.Descriptions of Data: Descriptive Statistics UNIT V Obtaining Reproducible Laboratory Measurements. Chapter 15.Introduction to Instrumental Methods and Electricity. Chapter 16.The Measurement of Weight Chapter 17.The Measurement of Volume. Chapter 18.The Measurement of Temperature. Chapter 19.The Measurement of pH, Selected Ions, and Conductivity. Chapter 20.Measurements Involving Light – Part A: Basic Principles and Instrumentation UNIT VI Laboratory Solutions. Chapter 21.Preparation of Laboratory Solutions – Part A: Concentration Expressions and Calculations. Chapter 22.Preparation of Laboratory Solutions – Part B: Basic Procedures and Buffers. Chapter 23.Solutions: Associated Procedures and Information. Chapter 24.Laboratory Solutions to Support the Activity of Biological Macromolecules UNIT VII Quality Assays and Tests. Chapter 25.Introduction to Quality Laboratory Tests and Assays. Chapter 26.Achieving Reproducible Results with Polymerase Chain Reaction Assays Chapter 27.Measurements Involving Light – Part B: Assays. Chapter 28.Achieving Reproducible Immunoassay Results. UNIT VIII Cell Culture and Reproducibility Chapter 29.Introduction to Quality Practices for Cell Culture. Chapter 30.Culture Media for Intact Cells. Culture and Reproducibility Chapter 31.Introduction to Quality Practices for Cell Culture Chapter 32.Culture Media for Intact Cells UNIT IX Basic Separation Method Chapter 33.ntroduction to Filtration. Chapter 34.Introduction to Centrifugation Chapter 35.Introduction to Bioseparations UNIT X Biotechnology and Regulatory Affairs. Chapter 36.Biotechnology and the Regulation of Medical and Food Products. Chapter 37.The Lifecycles and Regulation of Pharmaceutical Products. Chapter 38.The Lifecycles and Regulation of Biopharmaceutical and Regenerative Medicine Products. Chapter 39.Quality Systems in a Regulated Production Facility. Appendix: Answers to Practice Problems. Acronyms. Glossary Terms. En ligne : https://images.routledge.com/common/jackets/crclarge/978036724/9780367244880.jpg

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Laboratory manual for biotechnology and laboratory science:The basics,Revises edition / Lisa A.Seidman  

Public ISBD Titre : Laboratory manual for biotechnology and laboratory science:The basics,Revises edition Type de document : texte imprimé Auteurs : Lisa A.Seidman, Auteur ; Mary Ellen Kraus, Auteur ; Diana Lietzke Brandner, Auteur ; Jeanette Mowery, Auteur Editeur : CRC Press Année de publication : 2023 Importance : 422p. Présentation : ill. Format : 28 cmx21cm ISBN/ISSN/EAN : 978-1-03-241991-6 Langues : Anglais (eng) Langues originales : Anglais (eng) Index. décimale : 660 Technologie chimique (chimie industrielle) et techniques connexes Résumé : . Provides the basic laboratory skills and knowledge to pursue a career in biotechnology. Written by four biotechnology instructors with over 20 years of teaching experience, it incorporates instruction, exercises, and laboratory activities that the authors have been using and perfecting for years. These exercises and activities help students understand the fundamentals of working in a biotechnology laboratory. Building skills through an organized and systematic presentation of materials, procedures, and tasks, the manual explores overarching themes that relate to all biotechnology workplaces including forensic, clinical, quality control, environmental, and other testing laboratories. Features: • Provides clear instructions and step-by-step exercises to make learning the material easier for students. • Emphasizes fundamental laboratory skills that prepare students for the industry. • Builds students’ skills through an organized and systematic presentation of materials, procedures, and tasks. • Updates reflect recent innovations and regulatory requirements to ensure students stay up to date. • Supplies skills suitable for careers in forensic, clinical, quality control, environmental, and other testing laboratories. Note de contenu : . Contente: Unit i safety in the laboratory Unit Introduction Safety part 1: creating a safe workplace Fundamental Principles Classroom Activity 1: Performing a Risk Assessment Classroom Activity 2: Exploring Safety-Related Government Web Sites Classroom Activity 3: Responding to Emergencies Safety part 2: working safely with chemicals Fundamental Principles Classroom Activity 4: Understanding the Chemicals with Which You Work Classroom Activity 5: Personal Protection Laboratory Exercise 1: Tracking the Spread of Chemical Contamination Classroom Activity 6: Analyzing Safety Issues in a Laboratory Procedure Safety part 3: working safely with biological hazards Fundamental Principles Laboratory Exercise 2: Production of Bioaerosols and Factors Affecting Aerosol Production Unit Discussion Safety Contract, student copy Safety Contract, to turn in Unit ii documentation in the laboratory Unit Introduction Classroom Activity 7: Being an Auditor Laboratory Exercise 3: Keeping a Laboratory Notebook Classroom Activity 8: Writing and Following an SOP Unit Discussion Unit iii: metrology in the laboratory Unit Introduction Laboratory Exercise 4: Recording Measurements with the Correct Number of Significant Figures Classroom Activity 9: Constructing a Simple Balance Laboratory Exercise 5: Weight Measurements 1: Good Weighing Practices Laboratory Exercise 6: Weight Measurements 2: Performance Verification Laboratory Exercise 7: Volume Measurements 1: Proper Use of Volume Measuring Devices Laboratory Exercise 8: Volume Measurements 2: Performance Verification of a M Micropipette Laboratory Exercise 9: Measuring pH with Accuracy and Precision Unit Discussion Unit iv :spectrophotometry and the measurement of light Unit Introduction Laboratory Exercise 10: Color and the Absorbance of Light Laboratory Exercise 11: Concentration, Absorbance, and Transmittance Laboratory Exercise 12: Preparing a Standard Curve With Food Coloring and Using it for Quantitation Classroom Activity 10: Beer’s Law and Calculating an Absorptivity Constant Laboratory Exercise 13: Determination of the Absorptivity Constant for ONP Unit Discussion Unit v: biological solutions Unit Introduction Classroom Activity 11: Getting Ready to Prepare Solutions with One Solute: Calculations Classroom Activity 12: Getting Ready to Prepare Solutions with One Solute: Ordering Chemicals Laboratory Exercise 14: Preparing Solutions with One Solute Laboratory Exercise 15: Preparing Solutions to the Correct Concentration Laboratory Exercise 16: Working with Buffers Laboratory Exercise 17: Preparing Breaking Buffer Laboratory Exercise 18: Preparing TE Buffer Laboratory Exercise 19: More Practice Making a Buffer Laboratory Exercise 20: Making a Quality Product in a Simulated Company Unit Discussion UNIT VI ASSAYS Unit Introduction Laboratory Exercise 21: Two Qualitative Assays Laboratory Exercise 22: UV Spectrophotometric Assay of DNA: Quantitative Application Laboratory Exercise 23: UV Spectrophotometric Assay of DNA and Proteins: Qualitative Applications Laboratory Exercise 24: The Bradford Protein Assay: Learning the Assay Laboratory Exercise 25: The Bradford Protein Assay: Exploring Assay Verification Laboratory Exercise 26: The Beta-Galactosidase Enzyme Assay Laboratory Exercise 27: Comparing the Specific Activity of Two Preparations of Beta-Galactosidase Laboratory Exercise 28: Using Spectrophotometry for Quality Control: Niacin Unit Discussion UNIT VII BIOLOGICAL SEPARATION METHODS Unit Introduction Classroom Activity 13: Planning for Separating Materials Using a Centrifuge Laboratory Exercise 29: Separation of Two Substances Based on Their Differential Affinities for Two Phases Laboratory Exercise 30: Separation and Identification of Dyes Using Paper Chromatography Laboratory Exercise 31: Separating Molecules by Agarose Gel Electrophoresis Laboratory Exercise 32: Using Agarose Gel Electrophoresis to Perform an Assay Laboratory Exercise 33: Optimizing Agarose Gel Electrophoresis Laboratory Exercise 34: Quantification of DNA by Agarose Gel Electrophoresis Laboratory Exercise 35: Introduction to Ion Exchange Column Chromatography Unit Discussion Unit viii :growing cells Unit Introduction Growing cells part i: bacterial cells Laboratory Exercise 36: Using a Compound Light Microscope Laboratory Exercise 37: Aseptic Technique on an Open Lab Bench Laboratory Exercise 38: Working with Bacteria on an Agar Substrate: Isolating Individual Colonies Laboratory Exercise 39: Gram Staining Laboratory Exercise 40: Preparing Phosphate-Buffered Saline Laboratory Exercise 41: The Aerobic Spread-Plate Method of Enumerating Colony- Forming Units Laboratory Exercise 42: Preparing a Growth Curve for E. coli Growing cells part 2: mammalian cells Laboratory Exercise 43: Aseptic Technique in a Biological Safety Cabinet Laboratory Exercise 44: Making Ham’s F12 Medium from Dehydrated Powder Laboratory Exercise 45: Examining, Photographing, and Feeding CHO Cells Laboratory Exercise 46: Counting Cells Using a Hemocytometer Laboratory Exercise 47: Subculturing CHO Cells Laboratory Exercise 48: Preparing a Growth Curve for CHO Cells Unit Discussion Appendices 1. Abbreviations and Acronyms Used in this Laboratory Manual 2. Glossary 3. Selected Bibliography 4. Brief Metric Review 5. Calculating Standard Deviation 6. Equipment, Supplies, and Reagents Required for Each Unit 7. Recipes and Preparation Notes En ligne : https://images.routledge.com/common/jackets/crclarge/978103241/9781032419916.jpg Laboratory manual for biotechnology and laboratory science:The basics,Revises edition [texte imprimé] / Lisa A.Seidman, Auteur ; Mary Ellen Kraus, Auteur ; Diana Lietzke Brandner, Auteur ; Jeanette Mowery, Auteur . - [S.l.] : CRC Press, 2023 . - 422p. : ill. ; 28 cmx21cm. ISBN : 978-1-03-241991-6 Langues : Anglais (eng) Langues originales : Anglais (eng) Index. décimale : 660 Technologie chimique (chimie industrielle) et techniques connexes Résumé : . Provides the basic laboratory skills and knowledge to pursue a career in biotechnology. Written by four biotechnology instructors with over 20 years of teaching experience, it incorporates instruction, exercises, and laboratory activities that the authors have been using and perfecting for years. These exercises and activities help students understand the fundamentals of working in a biotechnology laboratory. Building skills through an organized and systematic presentation of materials, procedures, and tasks, the manual explores overarching themes that relate to all biotechnology workplaces including forensic, clinical, quality control, environmental, and other testing laboratories. Features: • Provides clear instructions and step-by-step exercises to make learning the material easier for students. • Emphasizes fundamental laboratory skills that prepare students for the industry. • Builds students’ skills through an organized and systematic presentation of materials, procedures, and tasks. • Updates reflect recent innovations and regulatory requirements to ensure students stay up to date. • Supplies skills suitable for careers in forensic, clinical, quality control, environmental, and other testing laboratories. Note de contenu : . Contente: Unit i safety in the laboratory Unit Introduction Safety part 1: creating a safe workplace Fundamental Principles Classroom Activity 1: Performing a Risk Assessment Classroom Activity 2: Exploring Safety-Related Government Web Sites Classroom Activity 3: Responding to Emergencies Safety part 2: working safely with chemicals Fundamental Principles Classroom Activity 4: Understanding the Chemicals with Which You Work Classroom Activity 5: Personal Protection Laboratory Exercise 1: Tracking the Spread of Chemical Contamination Classroom Activity 6: Analyzing Safety Issues in a Laboratory Procedure Safety part 3: working safely with biological hazards Fundamental Principles Laboratory Exercise 2: Production of Bioaerosols and Factors Affecting Aerosol Production Unit Discussion Safety Contract, student copy Safety Contract, to turn in Unit ii documentation in the laboratory Unit Introduction Classroom Activity 7: Being an Auditor Laboratory Exercise 3: Keeping a Laboratory Notebook Classroom Activity 8: Writing and Following an SOP Unit Discussion Unit iii: metrology in the laboratory Unit Introduction Laboratory Exercise 4: Recording Measurements with the Correct Number of Significant Figures Classroom Activity 9: Constructing a Simple Balance Laboratory Exercise 5: Weight Measurements 1: Good Weighing Practices Laboratory Exercise 6: Weight Measurements 2: Performance Verification Laboratory Exercise 7: Volume Measurements 1: Proper Use of Volume Measuring Devices Laboratory Exercise 8: Volume Measurements 2: Performance Verification of a M Micropipette Laboratory Exercise 9: Measuring pH with Accuracy and Precision Unit Discussion Unit iv :spectrophotometry and the measurement of light Unit Introduction Laboratory Exercise 10: Color and the Absorbance of Light Laboratory Exercise 11: Concentration, Absorbance, and Transmittance Laboratory Exercise 12: Preparing a Standard Curve With Food Coloring and Using it for Quantitation Classroom Activity 10: Beer’s Law and Calculating an Absorptivity Constant Laboratory Exercise 13: Determination of the Absorptivity Constant for ONP Unit Discussion Unit v: biological solutions Unit Introduction Classroom Activity 11: Getting Ready to Prepare Solutions with One Solute: Calculations Classroom Activity 12: Getting Ready to Prepare Solutions with One Solute: Ordering Chemicals Laboratory Exercise 14: Preparing Solutions with One Solute Laboratory Exercise 15: Preparing Solutions to the Correct Concentration Laboratory Exercise 16: Working with Buffers Laboratory Exercise 17: Preparing Breaking Buffer Laboratory Exercise 18: Preparing TE Buffer Laboratory Exercise 19: More Practice Making a Buffer Laboratory Exercise 20: Making a Quality Product in a Simulated Company Unit Discussion UNIT VI ASSAYS Unit Introduction Laboratory Exercise 21: Two Qualitative Assays Laboratory Exercise 22: UV Spectrophotometric Assay of DNA: Quantitative Application Laboratory Exercise 23: UV Spectrophotometric Assay of DNA and Proteins: Qualitative Applications Laboratory Exercise 24: The Bradford Protein Assay: Learning the Assay Laboratory Exercise 25: The Bradford Protein Assay: Exploring Assay Verification Laboratory Exercise 26: The Beta-Galactosidase Enzyme Assay Laboratory Exercise 27: Comparing the Specific Activity of Two Preparations of Beta-Galactosidase Laboratory Exercise 28: Using Spectrophotometry for Quality Control: Niacin Unit Discussion UNIT VII BIOLOGICAL SEPARATION METHODS Unit Introduction Classroom Activity 13: Planning for Separating Materials Using a Centrifuge Laboratory Exercise 29: Separation of Two Substances Based on Their Differential Affinities for Two Phases Laboratory Exercise 30: Separation and Identification of Dyes Using Paper Chromatography Laboratory Exercise 31: Separating Molecules by Agarose Gel Electrophoresis Laboratory Exercise 32: Using Agarose Gel Electrophoresis to Perform an Assay Laboratory Exercise 33: Optimizing Agarose Gel Electrophoresis Laboratory Exercise 34: Quantification of DNA by Agarose Gel Electrophoresis Laboratory Exercise 35: Introduction to Ion Exchange Column Chromatography Unit Discussion Unit viii :growing cells Unit Introduction Growing cells part i: bacterial cells Laboratory Exercise 36: Using a Compound Light Microscope Laboratory Exercise 37: Aseptic Technique on an Open Lab Bench Laboratory Exercise 38: Working with Bacteria on an Agar Substrate: Isolating Individual Colonies Laboratory Exercise 39: Gram Staining Laboratory Exercise 40: Preparing Phosphate-Buffered Saline Laboratory Exercise 41: The Aerobic Spread-Plate Method of Enumerating Colony- Forming Units Laboratory Exercise 42: Preparing a Growth Curve for E. coli Growing cells part 2: mammalian cells Laboratory Exercise 43: Aseptic Technique in a Biological Safety Cabinet Laboratory Exercise 44: Making Ham’s F12 Medium from Dehydrated Powder Laboratory Exercise 45: Examining, Photographing, and Feeding CHO Cells Laboratory Exercise 46: Counting Cells Using a Hemocytometer Laboratory Exercise 47: Subculturing CHO Cells Laboratory Exercise 48: Preparing a Growth Curve for CHO Cells Unit Discussion Appendices 1. Abbreviations and Acronyms Used in this Laboratory Manual 2. Glossary 3. Selected Bibliography 4. Brief Metric Review 5. Calculating Standard Deviation 6. Equipment, Supplies, and Reagents Required for Each Unit 7. Recipes and Preparation Notes En ligne : https://images.routledge.com/common/jackets/crclarge/978103241/9781032419916.jpg

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